Using shed cell collectors requires standardized methods to ensure efficient cell recovery and sample integrity for forensic, research, and clinical applications. These procedures guide from pre-collection prep to post-collection handling, minimizing contamination and maximizing DNA yield.
Core Methods & Procedures
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Prep & Safety: Gather single-use collectors, gloves, preservative tubes, and labels. Put on fresh gloves; inspect collector packaging for damage.
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Surface Assessment: Identify target area (e.g., weapon grip, door handle); note surface type (porous/non-porous) to adjust pressure.
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Collection: For rigid collectors—press textured surface firmly, move in 5–10 circular motions. For swab-style—swab surface with moderate pressure, covering 4–6 cm².
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Sample Transfer: Immediately place collector into preservative tube (e.g., lysis buffer) or press onto FTA card; avoid touching sampling surface.
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Labeling & Documentation: Mark tube/card with ID, date, location, and collector; log in chain-of-custody records.
Key Features
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Contamination Control: Single-use design and glove protocols prevent cross-contamination.
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Cell Yield Optimization: Standardized pressure/movement ensures consistent, high cell recovery.
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Surface Adaptability: Works on porous (fabric, wood) and non-porous (metal, glass) surfaces.
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User-Friendly: Simple steps suitable for trained personnel in field or lab settings.
Scope & Applications
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Forensics: Crime scene evidence (weapons, clothing) for DNA profiling.
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Clinical Research: Collecting epithelial cells for disease biomarker studies.
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Environmental Monitoring: Recovering microbial cells from surfaces in healthcare facilities.