This article covers adhesion properties and usage tips for DNA collection pens, ensuring efficient, contamination-free trace DNA sampling in forensic and research settings.
Adhesion Properties
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Targeted Adhesion: Specialized tip material (e.g., hydrophilic foam) adheres to epithelial cells/trace DNA without absorbing excess debris.
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Controlled Release: Releases >95% collected DNA into extraction buffers, maximizing sample yield for analysis.
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Surface Versatility: Adheres effectively to non-porous (glass, metal) and semi-porous (fabric, wood) surfaces common in crime scenes.
Usage Tips
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Prep: Ensure pen tip is dry; avoid touching tip with gloves to prevent cross-contamination.
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Sampling: Apply light, consistent pressure; swipe tip in single direction (3–5 passes) to optimize adhesion.
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Transfer: Immediately place tip into sterile extraction tube; avoid prolonged exposure to air to prevent DNA degradation.
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Labeling: Mark tubes with sample ID, surface type, and collection date to maintain chain of custody.
Application Scope
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Forensic Crime Scenes: Sampling trace DNA from weapons, tools, or surfaces (e.g., doorknobs, clothing).
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Research Labs: Collecting epithelial cell samples for genetic studies or microbiome analysis.
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Biometric Testing: Non-invasive DNA sampling for identity verification in controlled environments.