This article highlights common mistakes when using saliva collection devices and key precautions, ensuring high-quality, contamination-free samples for research and diagnostics.
Common Mistakes
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Inadequate Sample Volume: Collecting less than the required amount (often 2–5mL) due to rushing or insufficient saliva stimulation.
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Contamination Risks: Touching the device’s collection tip with hands or lips, or using devices with damaged sterile packaging.
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Improper Preservation: Failing to add preservative buffer promptly or mixing buffer unevenly with saliva.
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Timing Errors: Collecting samples within 30 mins of eating/drinking/smoking, which introduces food residues or chemicals.
Key Precautions
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Prep: Fast (no food/drink/smoke) for 30 mins; stimulate saliva by chewing gum or tongue movement if needed.
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Collection: Avoid tip contact with any surfaces; fill to the device’s marked volume line.
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Preservation: Add buffer immediately after collection; invert 5–10 times to mix thoroughly.
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Handling: Label with sample ID/date; store at room temp (or as directed) and ship within recommended timeframes.
Application Scope
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Genetic Research: Saliva sample collection for ancestry, disease marker, or microbiome studies.
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Clinical Diagnostics: At-home or lab-based saliva testing for viral infections, hormone levels, or genetic disorders.
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Forensics: Saliva evidence collection from items like cups, cigarettes, or bite marks.