This article shares practical methods to boost DNA sample recovery rates, ensuring maximum yield while maintaining sample integrity for forensics and research.
Key Improvement Methods
-
Sampling Tool Selection: Use flocked swabs (higher surface area) over standard swabs; choose conical collectors for textured surfaces to access crevices.
-
Extraction Protocol Optimization: Adjust buffer volume (match sample size) and incubation time (15–20 mins for dried samples); use enzymatic lysis for tough cell types.
-
Contamination Prevention: Sterilize tools/workspaces, use single-use materials, and avoid glove contact with sampling tips to reduce sample loss.
-
Post-Sampling Handling: Transfer samples to extraction buffers immediately; store at 4°C if processing is delayed to prevent DNA degradation.
Method Features
-
Yield-Focused: Methods can increase recovery by 25–40% for low-abundance samples.
-
User-Friendly: No specialized equipment needed—adapts to standard lab/field workflows.
-
Integrity-Preserving: Avoids harsh treatments that damage DNA quality.
Application Scope
-
Forensics: Recovering trace DNA from crime scene evidence (weapons, surfaces).
-
Research Labs: Maximizing yield from limited biological samples (ancient DNA, clinical biopsies).
-
Diagnostics: Improving recovery from low-volume samples (saliva, nasal swabs) for genetic tests.