Standardized usage of micro-sample collection tools is critical for DNA labs to ensure accuracy, reproducibility, and sample integrity when handling small volumes (μL-scale) of biological material. These protocols govern tool selection, handling, and processing to minimize errors and contamination.
Core Standardized Procedures
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Tool Preparation: Use sterile, certified micro-tools (pipette tips, micro-swabs, capillary tubes); inspect for defects and ensure compatibility with sample type (DNA, RNA).
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Sample Collection: Calibrate pipettes to 1–100μL volumes; use micro-swabs for solid surfaces, capillary tubes for liquid samples—apply consistent pressure/volume to avoid over/under-sampling.
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Contamination Control: Change gloves between samples; use filter tips to prevent aerosol carryover; dedicate work zones for pre- and post-amplification steps.
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Processing & Storage: Transfer samples to labeled microcentrifuge tubes with preservatives; store at -20°C/-80°C; document all steps in lab logs.
Key Features
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Precision: Tools with sub-microliter accuracy ensure consistent sample volumes.
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Traceability: Barcoded tools and digital logging enable full sample lifecycle tracking.
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Compliance: Aligns with ISO 17025, CLIA standards for lab accreditation.
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Efficiency: Optimized workflows reduce sample loss and processing time.
Scope & Applications
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Forensic DNA Labs: Processing trace evidence (saliva, touch DNA) with limited material.
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Clinical Genetics: Analyzing patient micro-samples (buccal swabs, plasma) for genetic testing.
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Research Labs: Handling precious samples (ancient DNA, single-cell samples) for sequencing.