The Enhanced Double-Hole Blood Sample Collection Card Kit is an advanced solution designed to streamline and improve the efficiency of blood sample collection, preservation, and downstream analysis. Building on the foundational benefits of traditional dried blood spot (DBS) cards, this enhanced kit features a dual-hole design that enables simultaneous collection of two independent blood samples on a single card—addressing the need for duplicate testing, multi-assay compatibility, or backup samples in clinical, forensic, and research settings. Integrated with upgraded preservation reagents and user-centric accessories, the kit ensures long-term DNA/biomarker stability while simplifying workflow for field and lab personnel alike.
Kit Composition & Design Features
The kit is thoughtfully engineered with components that prioritize sample integrity, operational ease, and versatility:
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Double-Hole Collection Card: A cellulose-based card with two distinct, labeled sample wells (5–8mm diameter each) separated by a hydrophobic barrier. This prevents cross-contamination between samples while allowing side-by-side collection. The card is impregnated with enhanced preservation reagents—including chaotropic salts, EDTA, and antimicrobial agents—that lyse cells, inactivate nucleases, and inhibit microbial growth to preserve DNA and proteins for up to 8 years at room temperature.
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Sample Application Tools: Sterile capillary tubes (10μL/20μL) for precise blood volume measurement, disposable transfer pipettes, and a calibrated dropper to ensure consistent sample loading into each hole. The tools reduce human error and ensure uniform sample spreading within each well.
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Preservation & Storage Accessories: Moisture-resistant foil pouches with pre-loaded silica gel desiccants to maintain card dryness during storage/transport, tamper-evident seals for chain-of-custody tracking, and a waterproof storage box to protect cards from environmental damage.
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Documentation & Safety Supplies: Printed labels for sample identification (patient ID, date, time), chain-of-custody forms, disposable nitrile gloves, and absorbent pads for spill cleanup—supporting compliance and user safety.
Key Functional Advantages
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Dual-Sample Efficiency: Eliminates the need for multiple single-hole cards by enabling two independent samples per card, reducing kit costs and storage space by up to 50%.
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Cross-Contamination Prevention: Hydrophobic barriers between holes and sterile application tools ensure no sample mixing, critical for duplicate testing or multi-analyte workflows (e.g., DNA genotyping and infectious disease screening).
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Enhanced Biomarker Stability: Upgraded reagents improve preservation of both DNA and proteins, expanding compatibility with downstream applications like PCR, ELISA, and next-generation sequencing (NGS).
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Field-Friendly Workflow: No refrigeration required, lightweight packaging (150–200g per kit), and clear, pictorial instructions make it suitable for remote or resource-limited settings.
Scope & Applications
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Clinical Diagnostics: Pediatric or geriatric blood sampling for duplicate genetic testing (e.g., newborn screening, rare disease diagnostics) or simultaneous infectious disease and biomarker analysis.
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Forensic Science: Collection of blood evidence from crime scenes for parallel DNA profiling (one sample for analysis, one for backup) while maintaining chain-of-custody integrity.
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Epidemiological Research: Large-scale surveys in remote regions, where dual samples support both immediate testing and long-term biobanking for future studies.
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Animal Health & Veterinary Science: Collection of small blood volumes from livestock or wildlife for concurrent pathogen detection and genetic diversity analysis.
Usage Best Practices
To maximize performance: Apply 10–20μL of blood to each hole (avoid overloading to prevent barrier breach); air-dry the card horizontally for 60–90 minutes before sealing in desiccated pouches; store at 15–25°C away from direct sunlight; and use a sterile punch tool to extract sample discs from each hole for downstream analysis.